Green Fluorescent Protein

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From:"Merriweather, Annie (OD/ORS)" <>
To:"''" <>
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I'm looking for help with the best fixation/processing methodologies for
histology of tissues expressing fluorescence from Enhanced Green Fluorescent
Protein (a Clontech product).  

An investigator doing gene-transfer research has transplanted autologous
bone marrow that had been transfected with the GFP gene into a rhesus
monkey.  He wants to be able to take biopsies from the monkey, fix the
tissue, embed and section it for fluorescence microscopy.  Alcohols
apparently kill the signal -
which makes embedding problematic.  

Has anyone successfully done fluorescence microscopy on fixed tissue
sections which demonstrate which cells are expressing the GFP? 
Thanks in advance your help. 

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