Green Fluorescent Protein
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From: | "Merriweather, Annie (OD/ORS)" <merriweath@vrp.ncrr.nih.gov> |
To: | "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
I'm looking for help with the best fixation/processing methodologies for
histology of tissues expressing fluorescence from Enhanced Green Fluorescent
Protein (a Clontech product).
An investigator doing gene-transfer research has transplanted autologous
bone marrow that had been transfected with the GFP gene into a rhesus
monkey. He wants to be able to take biopsies from the monkey, fix the
tissue, embed and section it for fluorescence microscopy. Alcohols
apparently kill the signal -
which makes embedding problematic.
Has anyone successfully done fluorescence microscopy on fixed tissue
sections which demonstrate which cells are expressing the GFP?
Thanks in advance your help.
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