Re: PH of IHC buffers

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From:Joyce Kotzuk <JKotzuk@salud.unm.edu>
To:azdudley@hotmail.com, histonet@pathology.swmed.edu, settembr@UMDNJ.EDU
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I calibrate my pH meter once a day.  Is this overkill? I do it because that's what was done in my old lab, not because I know for sure that's what needs to be done. How often do you all out there calibrate? Thanks,
Joyce Kotzuk, UNM pathology dept.

>>> Dana Settembre <settembr@UMDNJ.EDU> 03/30/00 11:30AM >>>
On Wed, 29 Mar 2000, anita dudley wrote:

> Would anyone out there let me know what they use to check the ph of their 
> IHC buffer.  It is a question on the CAP checklist, we have dip sticks but I 
> just wonder if other people are using a ph meter and what kind and is it 
> checked every day?  thanks so much for the info
> 
> Anita Dudley
> Providence Hosp
> Mobile Ala
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> 
Hi Anita,
I use a pH meter by Corning.  I make buffer about once or twice a week.  I
pH each time I make it.  I calibrate my pH meter once a month.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA






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