Re: PH of IHC buffers
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From: | Joyce Kotzuk <JKotzuk@salud.unm.edu> |
To: | azdudley@hotmail.com, histonet@pathology.swmed.edu, settembr@UMDNJ.EDU |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
I calibrate my pH meter once a day. Is this overkill? I do it because that's what was done in my old lab, not because I know for sure that's what needs to be done. How often do you all out there calibrate? Thanks,
Joyce Kotzuk, UNM pathology dept.
>>> Dana Settembre <settembr@UMDNJ.EDU> 03/30/00 11:30AM >>>
On Wed, 29 Mar 2000, anita dudley wrote:
> Would anyone out there let me know what they use to check the ph of their
> IHC buffer. It is a question on the CAP checklist, we have dip sticks but I
> just wonder if other people are using a ph meter and what kind and is it
> checked every day? thanks so much for the info
>
> Anita Dudley
> Providence Hosp
> Mobile Ala
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Hi Anita,
I use a pH meter by Corning. I make buffer about once or twice a week. I
pH each time I make it. I calibrate my pH meter once a month.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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