Dear Histonetters

Histonetters,

we are getting too much freeze thawing arefact in our mouse brain
cryosections.  Brains were fixed in 4% paraformaldehyde, then held in
sucrose.  We snap froze in isopentane on dry ice and transferred
to the cryostat for sectioning.  Any suggerstions please?  Should we go
directly from sucrose to embed on cryostat and section?.  We are also
considering freezing the brain slices (1-2 mm thick) in isopentane in a
Shandon
histobath, where temp is -50 C and not as cold as dry ice.  


thankyou

Dr Robert G. Russell
Director
Histotechnology and Comparative Pathology
Forchheimer  734
Phone: (718) 430-3209
Fax:   (718) 430-3243
E-mail:  russell@aecom.yu.edu

mail address:
Ullmann Building, Room 1005,
Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Av., Bronx, NY, 10461.