Re: Fixatives for Avian Tissue
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From: | Tony Henwood <henwood@mail.one.net.au> |
To: | histonet@pathology.swmed.edu, Gwin Kerce <gkerce@arches.uga.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Dear Gwin,
> Our lab is working on a project that involves locating calcium deposits
> in avian testis. We have tried to use Bouin's Solution, unfortunately,
> it dissolves the deposits of interest. We also tried 10% Buffered
> Foramlin, however, the ultrastructure was not preserved. Does anyone
> have any suggestions for a fixative? We need to fix both the testicular
> and epididmyal tissue.
>
Try Millonig's Buffered formalin. It give ultrastructural features
very close to those obtained with gluteraldehyde.
Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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