Re: Fixatives for Avian Tissue

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From:Tony Henwood <>, Gwin Kerce <>
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Dear Gwin,

> Our lab is working on a project that involves locating calcium deposits
> in avian testis.  We have tried to use Bouin's Solution, unfortunately,
> it dissolves the deposits of interest.  We also tried 10% Buffered
> Foramlin, however, the ultrastructure was not preserved.  Does anyone
> have any suggestions for a fixative?  We need to fix both the testicular
> and epididmyal tissue.
Try Millonig's Buffered formalin. It give ultrastructural features 
very close to those obtained  with gluteraldehyde.

Regards, Tony

Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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