Johns Hopkins eosin formula

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László G. Kömüves, Ph.D. [hope all those accents and umlauts get through AOL 
for Mac] at COR Therapeutics in South San Francisco CA asks for the old Johns 
Hopkins eosin mixture, which I posted on this list several years ago.

Bob Richmond
Samurai Pathologist
Knoxville TN

PURPOSE:  To describe the rather well-known but poorly documented eosin 
mixture used at Johns Hopkins Hospital in the 1960's and 70's.


Mix together

Eosin Y (C.I. 45380)      3.6 g
Phloxine B (C.I. 45410)      1.5 g
Biebrich scarlet (C.I. 26905 )   0.3 g
absolute alcohol    150   mL
distilled water   120   mL

Shake or stir with a magnetic stirrer to dissolve, then add 450 mL more of 
distilled water.

STAIN: About 3 minutes for a routine H & E stain. Frozen sections are stained 
almost instantaneously. Dehydrate without returning to water, clear, and 

RESULT: This eosin mixture gives an extremely bright eosin stain, not to 
every pathologist's taste. Myelin sheaths are very clearly demonstrated after 
routine fixation.


Donald Myers, chief histotechnologist, Department of Pathology, Johns Hopkins 
Hospital, about 1968. [He died in the early 1990's.] He may have invented 
this stain mixture, which has probably never been formally published. 
Procedure written by Robert S. Richmond, M.D., F.C.A.P. 

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