Moue brain perfusion
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From: | "BrosnanWatters, Gayle" <GBrosnanWatters@vanguard.edu> |
To: | 'histonet' <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="windows-1252" |
Peter,
I perfuse mouse brain with a paraformaldahyde and glutaraldehyde
solution, but I don't use any vasodilators. As I understand it, it depends
on what you are going to look for in them. Some procedures can't have
heparin (which is what my old lab at Wash U used for some stuff), and some,
of course, can't have glutaraldehyde, either. I am doing a simple stain on
mine, after embedding in araldite, and sectioning at 1 micron for light
microscopy.
I've got a recipe, but it may be not at all what you are looking for
- hey, I'm still just a psychologist, what do I know?
Gayle Brosnan-Watters, Ph.D.
Assistant Professor
Psychology Department
Vanguard University of Southern California
55 Fair Drive
Costa Mesa, CA 92626
Phone 714-556-3610 Ext. 454
Fax 714-966-6316
GBrosnanwatters@vanguard.edu
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