Moue brain perfusion

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From:"BrosnanWatters, Gayle" <>
To:'histonet' <>
Content-Type:text/plain; charset="windows-1252"

	I perfuse mouse brain with a paraformaldahyde and glutaraldehyde
solution, but I don't use any vasodilators.  As I understand it, it depends
on what you are going to look for in them.  Some procedures can't have
heparin (which is what my old lab at Wash U used for some stuff), and some,
of course, can't have glutaraldehyde, either.  I am doing a simple stain on
mine, after embedding in araldite, and sectioning at 1 micron for light
	I've got a recipe, but it may be not at all what you are looking for
- hey, I'm still just a psychologist, what do I know?
Gayle Brosnan-Watters, Ph.D.
Assistant Professor
Psychology Department
Vanguard University of Southern California
55 Fair Drive
Costa Mesa, CA  92626
Phone 714-556-3610 Ext. 454
Fax 714-966-6316

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