> -----Original Message-----
> From:	Reynolds, David  A 
> Sent:	Saturday, April 08, 2000 12:39 AM
> To:	'ToniT41@aol.com'
> Subject:	RE: Negative Controls for Immunohistochemistry
> 
> I use a known positive for a negative control, using a negative serum in
> place of the primary antibody.  On another slide, I run a patient section
> using a negative serum in place of the primary antibody.  I label then as
> Negative Control and Negative Patient.  (This is using the Sequenza
> coverplate system).
> 
> Dave Reynolds, HT(ASCP)
> Alaska Native Medical Center
> Anchorage, Alaska  99508
> 
> 
> -----Original Message-----
> From:	ToniT41@aol.com [SMTP:ToniT41@aol.com]
> Sent:	Thursday, April 06, 2000 12:03 PM
> To:	histonet@pathology.swmed.edu
> Subject:	Negative Controls for Immunohistochemistry
> 
> I would like to know what everyone is using for negative controls?
> 
> For example are you using a known positive control slide for mouse or
> rabbit 
> negative antibody?
> or known negative slides against the antibody itself?
> and is everyone using the patient control with mouse or rabbit negative 
> antibody?
> 
> I guess I'm trying to find out how labs are handleing negative controls?
> 
> Thanks
> Toni