protein concentration needed for IHC
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From: | Marcia Bentz <mb7x@virginia.edu> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 30 Sep 1999 08:30:51 -0400 |
Content-Type: | text/plain; charset="us-ascii" |
Hi All,
I was wondering if anyone has ever experimented with what protein
concentrations are detectable in IHC. My problem: an ELISA performed on
lysed supernatent from the same tissue I'm staining showed "off the scale"
for TNF alpha levels, measured in pg/ml. My TNF IHC shows just about nill
in terms of staining. I'm pretty much assuming it varies for each antibody,
but does anyone know a general baseline?
Thanks for the great help!
As always....
Marcia
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