protein concentration needed for IHC

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From:Marcia Bentz <mb7x@virginia.edu>
To:histonet@pathology.swmed.edu
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Date:Thu, 30 Sep 1999 08:30:51 -0400
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Hi All,
I was wondering if anyone has ever experimented with what protein
concentrations are detectable in IHC. My problem: an ELISA performed on
lysed supernatent from the same tissue I'm staining showed "off the scale"
for TNF alpha levels, measured in pg/ml. My TNF IHC shows just about nill
in terms of staining. I'm pretty much assuming it varies for each antibody,
but does anyone know a general baseline?
Thanks for the great help!
As always....

Marcia




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