mast cells
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From: | Mike Bromley <MBromley@PICR.man.ac.uk> |
To: | "'histonet'" <histonet@pathology.swmed.edu> |
Reply-To: | |
Date: | Wed, 22 Sep 1999 08:28:05 +0100 |
Content-Type: | text/plain; charset="us-ascii" |
Dear All,
Before available antibodies to mast cell enzymes I used a method I got
from Lung T. Yam using the enzyme substrate naphthol AS amino
caproate. The enzyme histochemistry worked on paraffin sections,
indeed I used it on glycol methacrylate sections, it was very
selective for mast cells. I found it very useful to differentiate
between PMNs and mast cells at sites of cartilage erosion in
rheumatoid arthritis (I used naphthol AS -D chloroacetate to stain for
PMNs and this substrate stains mast cells as well) you could visualise
apparently extracellular mast cell granules in the region. Originally
I got the substrate as a gift from Lung, but later I managed to get
some substrate made by a pharmaceutical company. I remember that a lot
of enzyme histochemistry was species specific in the sense that it
worked in some mammals not others.
L T Yam et al Eosinophilia in systemic mastocytosis. Am J Clin
Pathol vol 73:48-54 (1980)
M Bromley and DE Woolley Histopathology of the rheumatoid lesion
.Arthritis and Rheumatism vol 27 #8 857-863 (1984)
Mike Bromley
Histology Department
Paterson Institute
Wilmslow Road
Manchester
M20 9BX
UK
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