eosin blotchy problems

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
Date:Fri, 17 Sep 1999 09:43:38 -0600

You need to increase your rinsing times in running tap water, in order
to obtain the correct pH for the eosin.  Try 1 min after hematoxylin,
1 min after acid step, and 1 min after the bluing, then go to 
a lower alcohol % before your eosin, 70% or match the alcohol
concentration of your eosin solvent, if it is 50%, use 50% alcohol
before going into the eosin.

Gayle Callis

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