cytoplasm loss in frozens

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From:Gayle Callis <>
Date:Tue, 21 Sep 1999 10:41:42 -0600

For formalin fixation on frozens for an H&E, section at -17C, and place 
directly into NBF, RT for 1 min, sections do stay on slide, if you proceed
with IHC (cold fix, time determined by success of staining), rinse
with PBS.  For an H&E, I rinsed very gently in water post NBF fix,
 proceeded with H&E.

For any acetone fixation for IHC, air dry for 30 min up to 4 hours, 
fix cold for 10 min, air dry, store or rinse OCT away with PBS.  

Gayle Callis

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