On Thu, 16 Sep 1999, Donna Sitrin wrote:

> Does anyone know of any marking pen out there that will withstand methanol.

  How about a child's wax crayon? On a coverslip it might be 
  less easy to use than a liquid-emitting pen, but perhaps you 
  could collect the sections or cells onto slides rather than
  coverslips. I haven't tested crayon wax to see if it's
  methanol-proof. It's certainly waterproof, and waxes generally
  don't dissolve in methanol. You might need to do a few obvious
  tests to find a crayon colour that isn't extracted into methanol. 
  Extracted colorant might interfere with immunofluorescence
  methods. 

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1
   E-mail: kiernan@uwo.ca