Re: Stain

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From:Heike Grabsch <h.grabsch@uni-koeln.de>
To:histonet@pathology.swmed.edu, Ilhan Altinok <altinil@mail.auburn.edu>
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Date:Mon, 27 Sep 1999 22:19:20 -0700
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Hi Ilhan,

if you have formalin precipitates in your tissue sections you can use 
polarized light: it is birefringent (shining white), hemosiderin is not 
birefringent. So, you do not need a special stain. If you really think 
you need some staining: just do an iron staining (Prussian blue f.ex.) 
and you get a blue colour for hemosiderin.
Hope this helps. 

Dr. Heike Grabsch, Dept. of Pathology, Duesseldorf, Germany

Ilhan Altinok wrote:
> 
> Hi,
> 
> Does anyone have a protocol to stain only hemosiderin or to distinguish
> hemosiderin from formalin precipitated artifact?
> 
> Thanx
> 
> Ilhan



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