Re: Stain
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From: | Heike Grabsch <h.grabsch@uni-koeln.de> |
To: | histonet@pathology.swmed.edu, Ilhan Altinok <altinil@mail.auburn.edu> |
Reply-To: | |
Date: | Mon, 27 Sep 1999 22:19:20 -0700 |
Content-Type: | text/plain; charset=us-ascii |
Hi Ilhan,
if you have formalin precipitates in your tissue sections you can use
polarized light: it is birefringent (shining white), hemosiderin is not
birefringent. So, you do not need a special stain. If you really think
you need some staining: just do an iron staining (Prussian blue f.ex.)
and you get a blue colour for hemosiderin.
Hope this helps.
Dr. Heike Grabsch, Dept. of Pathology, Duesseldorf, Germany
Ilhan Altinok wrote:
>
> Hi,
>
> Does anyone have a protocol to stain only hemosiderin or to distinguish
> hemosiderin from formalin precipitated artifact?
>
> Thanx
>
> Ilhan
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