Re: Goldner's Trichrome Stain

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From:RSRICHMOND@aol.com
To:histonet@pathology.swmed.edu
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Date:Thu, 30 Sep 1999 18:27:49 EDT
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In a message dated 9/30/99 12:55:48 PM, Marianne Osborne in Omaha NE wrote:

<<Is there anyone out there in Histoland who has reference for the Goldner's
Trichrome Stain?

I am working tissues and slides for the class sets for the freshman medical 
students and have some old breast slides that say for the stain "Goldner's 
Trichrome". There is a reference to Villanueva-Goldner stain for 
plastic-embedded sections of bone but I will staining breast tissue.  

Thank you.  Marianne in Omaha, Ne.>>

This really takes this elderly pathologist back there. The stain requested is 
probably the green trichrome stain that was in use at Cornell Medical Center 
(New York Hospital, on 68th Street on the Upper East Side) between the 1930's 
and the 1960's. It was one of a number of trichrome techniques used as a 
routine surgical pathology stain (instead of H & E) by some surgical 
pathologists in the 1930's - at Cornell by Chandler Foot, one of the founders 
of American surgical pathology, who was chief there from 1932 to 1948. Most 
of his life's work was destroyed by administrative whim in 1960, and when I 
was at New York Hospital in 1968 only a few teaching-set slides survived, 
faded by generations of carbon-arc projection. - Papanicolaou was at Cornell 
then, and I suspect that he developed the Pap stain from the Goldner-Foot 
trichrome.

The stain uses alum hematoxylin, fast green FCF or light green SF, a red 
component ("ponceau de xylidene"), and an orange keratin stain (orange G - 
usually didn't work). I have the formula, but it will me take a couple of 
days to get it.

I would think that any standard green trichrome would give satisfactory 
results for staining breast tissue for freshman histology students. If I were 
doing this, I'd want an elastic component also. - More important is to work 
with a surgical pathologist to get a piece of well fixed, quite normal breast 
tissue - this could take a few weeks.

Bob Richmond
Samurai Pathologist
Knoxville TN



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