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<br>Dear Marg,
<p>Your normal processing protocol should work for these fish. Of
course, this is dependent on the size/species of fish. If they are
medaka, trout (upswim & fry), guppy, mesquito, or zebra they will process
fine if they are smaller than 3 cm. There are some species of fish
that will be a bit more troublesome than others. We decalcify the
whole fish in Decalcifier II from Surgipath prior to processing.
Your routine H&E will suffice, and your normal slides (non coated,
non treated) will work. Just make sure they dry for at least 16 hours.
If you do not decalcify these fish before processing, you will lose a great
deal of quality during sectioning. We routinely do hundreds of fish
per week using the suggestions I mentioned earlier. If you follow
these simple suggestions, your slides will turn out beautifully!
If you have any further questions, please feel free to contact me directly.
<p>Sincerely,
<p>Tom Galati
<br>Histo-Scientific Research Laboratories
<br>107 Killmon Road
<br>P.O. Box 30
<br>Basye, VA 22810
<br>(540)856-2222
<br>fax: (540)856-2227
<br>histosci@shentel.net
<br>"Hagerty, Marjorie A." wrote:
<blockquote TYPE=CITE>I have someone who would like us to process, cut,
and stain "fish blocks"
<br>for them.
<p>Having never, ever worked with fish histology, I have some questions
for
<br>those of you who do:
<br>1. Would I be able to use the same processing protocol we use for our
human
<br>surgicals?
<br>2. Would I be able to stain them with our regular H&E protocol?
<br>3. Are they different/difficult to cut?
<p>Any information you could share with me would be greatly appreciated.
<p>Thanks!
<br>Marg Hagerty
<br>Supervisor, Anatomic Pathology
<br>Eisenhower Medical Center
<br>39-000 Bob Hope Drive
<br>Rancho Mirage, CA 92270
<br> </blockquote>
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