Re: Disappearing Cytoplasm!!

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From:"Tony Henwood" <>, Bruce Abaloz <>
Date:Tue, 21 Sep 1999 23:02:52 +0000
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Dear Bruce,

> Hi all....I'm a student here @ the Uni.of Melbourne & am freezing fresh liver & spleen (mouse), floating the OCT covered specimen in a boat in liquid nitrogen
> until OCT is a solid block then I drop it all into the liquid nitrogen. I store it @ -70C until ready to cut in the cryostat.I am using Histogrip slides & cut @ 6 microns @ -10C.I let it dry @ room t> anyone advise me on what "to do" or what I'm doing 
wrong. I've also tried Acetone fixation (10 min.@ RT) with the same result??????????? I look forward to your replies & THANKYOU in advance.........S> 
I would suggest that after sectioning, try immediate fixation (& 
storage) in methanol or Schoobridge fixative (5ml formalin in 35ml 
absolute ethanol) prior to H&E staining.

Regards .... Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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