we use a Bielschowsky silver stain, you can contact us if u need the
procedure.
utmck
knoxville, tennesse
423 544-9089

> -----Original Message-----
> From:	J. A. Kiernan [SMTP:jkiernan@julian.uwo.ca]
> Sent:	Tuesday, September 21, 1999 1:22 PM
> Cc:	Histonet (E-mail)
> Subject:	Re: stain for alzheimers
> 
> > i am looking for a stain to determine alzheimers disease.  would
> appreciate 
> > any suggestions from anybody out there.
> > thanks so much, anita
>  
>    One of Ferenc Gallyas's silver methods is currently much
>    favoured by neuropathologists because of its high sensitivity
>    and clean background. 
> 
>      Gallyas F & Wolf JR 1986. Metal-catalyzed oxidation renders
>        silver intensification selective. J Histochem Cytochem
>        34: 1667-1672.
> 
>    There is a modification for large (whole human brain sections):
> 
>      Braak H & Braak E 1991. Demonstration of amyloid deposits and
>        neurofibrillary changes in whole brain sections.  Brain
>        Pathol. 1: 213-216.
> 
>  John A. Kiernan,
>  Department of Anatomy & Cell Biology,
>  The University of Western Ontario,
>  LONDON,  Canada  N6A 5C1
>