RE: iodine stain

<< Previous Message | Next Message >>
From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:"'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>, "'McManis, Kathleen'" <kamcma@pahosp.com>
Reply-To:
Date:Tue, 21 Sep 1999 12:23:41 -0400
Content-Type:text/plain

Kathleen,

One has to wonder why?
This is an old museum technique, that may be found in Culling's "Cellular
Pathology Technique" 4th ed. PP 465 and 537.
Fresh tissue slices may be stained for 1-2 hours in either gram's or Lugol's
iodine. Amyloid deposits are deep mahogany brown, this will turn blue on
further treatment with 10% sulphuric acid. Alternately one may treat
formaldehyde-fixed tissue slices in Lugol's iodine containing 1% sulphuric
acid for 1-2 hours, amyloid will be deep brown.

Good luck

Bryan


> ----------
> From: 	McManis, Kathleen[SMTP:kamcma@pahosp.com]
> Sent: 	September 20, 1999 2:03 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	iodine stain
> 
> Hi!
> 
> I am looking for a iodine stain, used on fresh tissue for the staining of
> amyloid. Any help would be appreciated.  Thanks
> 



<< Previous Message | Next Message >>