RE: Pancreatic Amylase

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From:Brown Alexander <AlexB@nayrshire.scot.nhs.uk>
To:Fazooly@aol.com, HistoNet <HistoNet@pathology.swmed.edu>
Reply-To:
Date:Mon, 20 Sep 1999 11:57:00 +0100
Content-Type:text/plain

Hi Fran,
	If it's for digesting glycogen,  -  we use alpha amylase type VI-B :
from porcine pancreas ( cat No. A-3176 ) obtained from Sigma.
We use it as a 1% solution in distilled water, at room temperature, for 20
mins.
	Regards,
		Alex Brown
		Crosshouse Hospital
		Kilmarnock, Scotland.
 ----------
From: Fazooly@aol.com
To: histonet@pathology.swmed.edu
Subject: Pancreatic Amylase
Date: Saturday, September 18, 1999 1:23AM

Does anyone out there have a pancreatic amylase that works on paraffin
embedded tissue?  We've tried and can't seem to get it to work.  Your help
is
greatly appreciated.
Thanks!
Fran
Yale University



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