The Microsporidial vacuolated and non-vacuolated spores that are seen in
humans are particularly small (1 to 2 microns in diameter) and are
difficult to detect in a clinical setting because of their size, poor
response and unpredictability with routine histochemical staining
techniques. The only definitive method for diagnosis is electron microscopy
of small bowel biopsies and PCR. Oil immersion-light microscopy on paraffin
sections is aided with a Weber's modified trichrome, Giemsa (for parasites),
and a Quick Hot-Gram Chromotrope 2R stain. These are methods which often
allow identification of certain Microsporidia genera in stool, duodenal
fluids and biopsies. I would be glad to fax you a copy of any of the above
mentioned procedures.


Eric C. Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center

-----Original Message-----
From: Brody,Juanita X
To: 'Histonet'
Sent: 9/15/99 1:16 PM
Subject: Microsporidiia Stain

Does any one have a procedure  for microsporidia and or a modified
trichrome
stainfor microsporidia