RE: HU antibody

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>, "'Donna Sitrin'" <>
Date:Fri, 24 Sep 1999 11:00:10 -0400


This was a topic on Histonet back in January.
I include the pertinent reply.



Anti-Hu MAb 16A11 is a robust early marker of vertebrate 
neurogenesis.  The antigen(s) detected by MAb 16A11 are expressed 
concomitant with or shortly after neuronal terminal mitosis, and are 
expressed at high levels in neuron cell bodies.  The exclusive cell 
body expression of neuronal Hu proteins makes Anti-Hu MAb 16A11  
extremely useful for enumeration of early neurons (unlike 
neurofilament and neurofilament associated proteins).

MAb 16A11 is a mouse IgG2b  that binds specifically to a conserved 
peptide epitope present in most known members of the Hu family of 
vertebrate neuronal proteins.  Thus, MAb 16A11 binds to human proteins
HuD and HuDpro (alternatively spliced forms of the HuD gene product)
as well as HuC (the HuC gene product).  In addition, the epitope 
recognized by MAb 16A11 is present in the predicted amino acid
 sequences of HuCLS (an alternatively spliced form of the HuC gene 
product), and Hel-N1 (the gene product of the third member of the 
mammalian Hu gene family).  The only known Hu protein isoform that
does not contain the amino acid domain recognized by MAb 16A11 
is HuDmex, which is a minor constituent of the Hu proteins present in
 human brain.  Western blot immunoassays of human, mouse, avian 
and fish neural tissue extracts, and recombinant human HuD and HuC
 proteins were used to document the specificity of antibody binding to 
Hu proteins.  Particle Concentration Fluorescence Immunoassays of 
synthetic peptides were used to identify the 16A11 epitope.  
Immunohistochemical analyses of human, mouse, avian and fish 
tissues documented the tissue and developmental patterns of Hu 


Marusich, M. F., Furneaux, H. M., Henion, P., Weston, J. A. (1994).  
Hu neuronal proteins are expressed in proliferating neurogenic cells.  
J. Neurobiol. 25, 143-155.

To facilitate distribution of this reagent, an initial sample of MAb 
16A11 will be provided at no charge to any investigator who requests 
it.  However, additional samples require payment of $120 per 275 ug 
sample to recover production and distribution costs.  

For more information contact:

Michael Marusich, Ph.D.
Director Monoclonal Antibody Facility
Institute of Neuroscience
University of Oregon
Eugene, OR 97403-1254

ph:  541-346-5688
fax: 541-346-4548

> ----------
> From: 	Donna Sitrin[]
> Sent: 	September 24, 1999 9:54 AM
> To:
> Subject: 	HU antibody
> Thank you for the information on myogenin.
> Does anyone have any information on a "HU"
> antibody? 
> Thanks, 
> Donna in Denver
> Unipath, LLC

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