We have been able to use NeoMarker's CD57(clone NK1) at a 1:200 on our
Ventana immunostainer with or without a 10 minute HIER in 1mM EDTA(pH8.0)
for NBF-fixed tissue...B5 fixed bone marrow biopsies seem to require a 20
minute HIER. 

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI 
(608)265-6596
(608)263-1568(fax)

> -----Original Message-----
> From:	Louise Taylor [SMTP:louiset@mail.saimr.wits.ac.za]
> Sent:	Monday, September 27, 1999 7:33 AM
> To:	histonet@pathology.swmed.edu
> Subject:	CD57
> 
>  Hi everyone!!
> 
> Has someone out there had any luck with this antibody. It labels natural
> killer cells in Hodgkins Lymphoma. I've  recently tried it-- but is only
> focally positive--not all the natural killer cells around the
> Reed-Sternberg cells exhibit immunoreactivity.
> 
> Thanks in advance. 
> 
> Shehnaz Khan
> Anatomical Path (Research)
> SAIMR 
>