Human CD3 + CD45 paraffin [not made in mouse]

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From:"Vaughan, Mary" <>
To:"'histonet'" <>
Date:Wed, 15 Sep 1999 11:31:54 -0400

Dear Histonetters,
	A colleague of mine has engrafted human tumor cells and human
lymphocytes s.c. into SCID mice. Now he would like to stain paraffin
sections of tissue for the presence of human lymphocytes (T cells). Does
anyone have any information on  antibodies to stain human CD3 and/or
human CD45 that will work in paraffin? The antibody can't cross-react
with mouse tissue or lymphocytes (although SCID mice do not have T
cells), so he's looking for [anti-human] antibodies made in something
other than mouse. We know about the kits to biotinylate the primary, but
would rather see if an appropriate antibody is available, before we go
this route.

 Please send any responses directly to him at:

Best Regards,

Mary Vaughan HT (ASCP)
Roswell Park Cancer Institute
Elm + Carlton Sts.   CDC-121
Buffalo, NY 14263

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