GI's and processor problems
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From: | "Jennings-Siena, Debbie" <ds.jennings-siena@baylordallas.edu> |
To: | "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu> |
Reply-To: | |
Date: | Thu, 30 Sep 1999 11:52:53 -0500 |
Content-Type: | text/plain |
Please help me figure this out. We had a processor problem last night. Our
rotary open style processor messed up last night and all of the GI biopsies
got dunked in each solution twice. I pryed them out of the paraffin coated
basket and then made new cassettes. Removed the tissues and heated them for
10 minutes and then dunked them in 2 changes of xylene and 1 change of
absolute for 5 minutes each. Then we reprocessed starting in 95% alcohol,
100% alcohol and then xylene and paraffin. Today, the sections are
unreadable according to the pathologists. There is no nuclear detail and
the tissues are very eosinophilic. I am racking my brain trying to figure
this out. I decided that maybe some one with a clear head could help out.
We have had our slide stainer, our processor and our coverslipper all break
this week. So I am just trying to put out fires everywhere. Thanks for your
help.
Debbie Jennings-Siena
Baylor University Medical Center
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