Eosin
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From: | Stamp1129@aol.com |
To: | histoNet@pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 16 Sep 1999 18:41:59 EDT |
Content-Type: | text/plain; charset="us-ascii" |
Dear Histonetters
Occasionally we run into the problem of blotchy eosin in our H&E stain
line. Microscopically, it appears after staining, the eosin doesn't stain
blotches in all our tissue. This is not recurrent in every rack of slides
though. Our staining setup is:
Hematoxylin 5 minutes
rinse about 10 dips in running water
Acid Alcohol 2 quick dips
rinse about 10 dips
Scotts Tap water 10 - 20 dips
rinse 10-20 dips
100% alcohol 10 dips
eosin 1 minute
4 changes of 100% alcohol
4 changes xylene
Should we change to Ammonia water or Lithium Carbonate water instead of
Scotts or is there another reason blotchy eosin occurs in this setup? Any
and all suggestions would be appeciated. If you need more info let me know.
Jim
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