Dear all, HELP

I have just recently rejoined the list after two years of being without a
PC, and would like to ask for advice re CD4 staining in paraffin sections.
We bought CD4 Ab from NovoCastra and tried it with EDTA antigen retrieval as
per data sheet, result nuclear staining. After many attempts with a variety
of heating times and solutions, including Dako high pH retrieval solution as
recommended, nio improvement. One of my techs went to Keith Millers lab and
obtained wonderful staining, came back and has ben unable to repeat it. So,
any suggestions gratefully received.

Many thanks in advance
John Auld
Immunocytochemistry
Royal Free Hospital
London