re:Help!! - Procedure Source

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From:Leslie Ratkay <>
Date:Thu, 09 Sep 1999 11:19:51 -0700
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I don't know the Phenol/Glycerin one, but I was using a Formol/Glycerol
reprocessing method (sound pretty much the same)from Laboratory Methods in
Histotechnology Armed Forces Institute of Pathology, Ed. EB Prophet, B Mills, JB
Arrington, LH Sobin,, 1992, page 31; ISBN: 1-881041-00-X). This book also cites
a reference: Luna LG, ed. Methods for reprocessing dried tissue specimens.
HistoLogic. 1978;8(2):1.
Hope this helps,

>There is a procedure for rehydration/softening of desiccated tissues that
>involves soaking the dry tissue in a Phenol/Glycerin solution, followed by a
>long wash in running tap water.  When the tissue has softened and has a
>slight puffiness, it can be reprocessing on a short cycle.

>Many dry, hard, tissues have been salvaged this way.  It was used more
>frequently back in the old Technicon days. (I'm sure that there are some of
>us who still remember the basket of tissues hanging in the air overnight -
>It was always such an interesting start to the day).

>I've used it for years, but do not know where it originated.  It was one of
>those "goodies" that I picked up throughout the years. No doubt it was
>passed to me by a colleague since my version was hand written.   If there
>are references out there,  I would appreciate the information so I can write
>it "properly" for our procedure manual.


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