manual immunostainers

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From:Gayle Callis <>
Date:Mon, 13 Sep 1999 09:44:20 -0600

The Shandon coverplate holders are frequently used in this laboratory
but not the whole Sequenza setup, the holders are adequate, take up little
space, you can do a hundred slides if you want, each holder costs
around $160, coverplates can be reused, we do that ad nauseum, unless
they are scratched and must be carefully washed.

The amount of buffer is minimal!  10 slides/holder, for a whole 
run used approx 120 mls of buffer, and I have 6 holders and could run
60 slides at a time, not bad for hand methods.

For paraffin, it works nicely, you will need to determine how long
each antibody needs to be stained with substrate/chromogen development, once
determined, easy.  

Antigen retrieval and quenching endog peroxidase can be done before mounting
the slides, for paraffin sections to avoid bubbles.  

You can put holders in refrig, cold room or 37C incubators is desired.
For efficient, inexpensive staining devices, this was a godsend.

Gayle Callis

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