Hi Histonetters and IPOXers,

One of our pathologists has asked us to find a way to identify the presence
of ganglia in frozen sections of colon biopsies.  Colonoscopy is performed
to identify Hirschsprung's disease.  The idea is to sample sections of colon
and perform quick stains to access the presence or absence of ganglia.  I
don't know of any immunohistochemistry procedure that can be done within the
30-45 minutes time frame.  All of the biopsies would be assessed while the
patient is on the O.R. table.

So....does anyone out there know of a histochemical, enzyme or
immunohistochemical procedure that could be used?

Your help, as always, is greatly appreciated,

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568