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From:"marjorie lehman" <Marjorie.Lehman@unilever.com>
To:histonet <histonet@pathology.swmed.edu>
Date:Fri, 10 Sep 1999 11:21:42 -0400

I'd appreciate any thoughts/recommendations/tips/etc. from people doing TUNEL
staining on human skin.
Last year I did many, many mouse skin sections and got beautiful results. This
year, using the same Co's kit, same fixative, same processing, etc, I am
getting all kinds of strange results with human skin ( example: 2 sections of
tissue on the same slide: all nuclei stain on 1, no nuclei stain on the other).
The only thing that was changed is that the mouse skin was fixed + days,
whereas the human skin was fixed for approx. 16 hours.
I have diluted the TdT as recommended in the "Troubleshooting" hints with the
kit, but am still getting inconsistent results.  Arrrgh!!!!!
Marge Lehman

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