Mary,

There are two problems with this proposal

One is that the two clones may give different results. You'd better check 
that out before proceeding.

Two is that you will have to do a lot of work to get this new antibody 
working. Is it worth doing that? It may be that it will take as long to get 
it working right as to get the usual antibody!


Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043



----Original Message Follows----
From: "Vaughan, Mary" <Mary.Vaughan@RoswellPark.org>
To: 'histonet' <HistoNet@Pathology.swmed.edu>
Subject: p53
Date: Wed, 08 Sep 1999 10:05:40 -0400

Good Morning Everyone,
	I routinely use clone D07 for p53 in paraffin sections [formalin
fixed]. An investigator has some clone CM1 [also for p53] from the same
company that he wants me to use, rather than spending the big $$$ [and
waiting 2 weeks] for what he's used previously. The company is
Novacastra the #NCL-p53-CM1. Please, if you have HANDS ON experience
with THIS antibody, would you e-mail me?  Thanks

Best Regards,
Mary Vaughan HT (ASCP)
Roswell Park Cancer Institute
Elm + Carlton Sts.   CDC-121
Buffalo, NY 14263








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