Re: Soap deparaffinizing

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From:marsha r price <>
Date:Sat, 04 Sep 1999 00:35:35 -0500

I am merely sharing information to my fellow histonetters as we do on a
daily basis, that may be useful and economical. Many histonetters have
shared their techniques and recipes with me free of charge and I will do
the same for them. Again as I have stated this is a technique that has
been published in the Journal of applied Immunohistochemistry, and is
information that is available to all that wants it. I do not question
that Declere is patent pending, however, the knowledge is there for
anyone who wants it and I will provide my technique to anyone who
requests it as they have so generously done so for me in the past and I
welcome the challenge. I do not know the proprietary ingredients of
Declere, nor do I want to know. I can only share a tried and proven
published technique with my fellow histonetters.

Marsha Price

On Fri, 03 Sep 1999 17:57:34 -0500 cmcorp <> writes:
>Dear Histonetters:
>The formula for Declere is proprietary and confidential at this time 
>Declere is patent pending. It appears to be a matter of speculation by 
>Price as to its composition. 
>We at Cell Marque would invite comparison of results using whatever
>formulation one may care to mix. Our own experience suggests that
>concentration of reagents in the retrieval solutions is critical to 
>results obtained. 
>I would suggest that those who are interested, do there own studies
>comparing Declere versus M. Price's formulation. 
>Mike Lacey, M.D. 
>Medical Director 
>Cell Marque Corp.
>At 10:31 AM 9/3/99 -0500, you wrote:
>>You can just add a little soap in your Citrate Buffer if you would 
>>I have used Tween 20, but it works just as well without the soap if 
>>rinse with Dawn and Di water after your antigen retrieval step. That 
>>all DeClere is is Citrate Buffer and Soap. Make your own and save 
>>money. I will attach my procedure as an e-file when I get home, I 
>>do it from work.
>>Marsha Price
>>On Fri, 03 Sep 1999 08:00:32 -0400 (EDT) Tim Morken 
>>>A soap deparaffinizing technique was detailed about two years ago in 
>>>Medicine by a person in Sweden. What is the reference in J Appl 
>>>----Original Message Follows----
>>>From: marsha r price <>
>>>Subject: Re: RE: Declere
>>>Date: Thu, 02 Sep 1999 19:01:42 -0500
>>>There is an interesting article in Journal of Applied
>>>Immunohistochemistry, that describes a time saving technique in 
>>>can skip the deparaffinization step and go straight to the antigen
>>>retrieval solution. You can use any antigen retrieval  solution 
>>>Buffer is my favorite). It is the heat that in the antigen retrieval 
>>>(HIER) that is deparaffinizing the slides. You can remove any 
>>>paraffin by rinsing in Dawn dishwashing detergent and DI water after 
>>>antigen retrieval step. If you would like my procedure or any 
>>>me know.
>>>Marsha Price, HT,QIHC (ASCP)
>>>On Thu, 2 Sep 1999 08:54:41 +0100 "margaret blount"
>>><> writes:
>>> >Where does this interesting reagent come from? I would like to 
>>> >a whirl.
>>> >Thanks,
>>> >Margaret BLount
>>> >Unilever Research
>>> >Beds
>>> >
>>> >
>>> >-----Original Message-----
>>> >From:	Shotsberger-Gray, Wanda
>>> >[]
>>> >Sent:	Tuesday, August 31, 1999 7:39 PM
>>> >To:	'Histonet';
>>> >Subject:	RE: Declere
>>> >
>>> >Terry, We used this at Harris (and still do for some ABs).  It 
>>> >quite
>>> >well!  We have used both pressure cooker and Energy Beam 
>>> >protocols, both work well.  Give it atry.  I think you will 
>>> >Wanda Shotsberger
>>> >Harris Methodist Hospital
>>> >Fort Wort TX
>>> > ----------
>>> >From:
>>> >To: 'Histonet'
>>> >Subject: Declere
>>> >Date: Wednesday, August 25, 1999 7:41AM
>>> >
>>> >I have just been handed some information on a substance called
>>> >Declere which apparantley de-waxes, rehydrates and unmasks
>>> >antigens in formalin fixed paraffin section,all at the same time.
>>> >Has anyone used, or is using this for ICC?
>>> >Terry.
>>> >Terry Hacker,
>>> >Medical Research Council,
>>> >Harwell,
>>> >Oxfordshire,
>>> >U.K.
>>> >
>>>Marsha Price HT (ASCP), QIHC (ASCP)
>>>Get Your Private, Free Email at

Marsha Price HT (ASCP), QIHC (ASCP)

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