Dear Histonetters:
 
The formula for Declere is proprietary and confidential at this time since
Declere is patent pending. It appears to be a matter of speculation by Ms.
Price as to its composition. 

We at Cell Marque would invite comparison of results using whatever
formulation one may care to mix. Our own experience suggests that
concentration of reagents in the retrieval solutions is critical to the
results obtained. 

I would suggest that those who are interested, do there own studies
comparing Declere versus M. Price's formulation. 

Mike Lacey, M.D. 
Medical Director 
Cell Marque Corp.

At 10:31 AM 9/3/99 -0500, you wrote:
>Tim, 
>You can just add a little soap in your Citrate Buffer if you would like.
>I have used Tween 20, but it works just as well without the soap if you
>rinse with Dawn and Di water after your antigen retrieval step. That is
>all DeClere is is Citrate Buffer and Soap. Make your own and save mega
>money. I will attach my procedure as an e-file when I get home, I cannot
>do it from work.
>Marsha Price
>
>On Fri, 03 Sep 1999 08:00:32 -0400 (EDT) Tim Morken <timcdc@hotmail.com>
>writes:
>>Marsha,
>>
>>A soap deparaffinizing technique was detailed about two years ago in 
>>Lab 
>>Medicine by a person in Sweden. What is the reference in J Appl 
>>Immuno?
>>
>>
>>----Original Message Follows----
>>From: marsha r price <mprice26@juno.com>
>>To: Margaret.Blount@unilever.com
>>CC: WandaShotsberger-Gray@hmhs.com, Histonet@pathology.swmed.edu, 
>>T.Hacker@har.mrc.ac.uk
>>Subject: Re: RE: Declere
>>Date: Thu, 02 Sep 1999 19:01:42 -0500
>>
>>Margaret,
>>There is an interesting article in Journal of Applied
>>Immunohistochemistry, that describes a time saving technique in which 
>>you
>>can skip the deparaffinization step and go straight to the antigen
>>retrieval solution. You can use any antigen retrieval  solution 
>>(Citrate
>>Buffer is my favorite). It is the heat that in the antigen retrieval 
>>step
>>(HIER) that is deparaffinizing the slides. You can remove any 
>>residual
>>paraffin by rinsing in Dawn dishwashing detergent and DI water after 
>>the
>>antigen retrieval step. If you would like my procedure or any recipes 
>>let
>>me know.
>>
>>Marsha Price, HT,QIHC (ASCP)
>>
>>On Thu, 2 Sep 1999 08:54:41 +0100 "margaret blount"
>><Margaret.Blount@unilever.com> writes:
>> >Where does this interesting reagent come from? I would like to give 
>>it
>> >a whirl.
>> >Thanks,
>> >Margaret BLount
>> >Unilever Research
>> >Beds
>> >margaret.blount@unilever.com
>> >
>> >-----Original Message-----
>> >From:	Shotsberger-Gray, Wanda
>> >[SMTP:WandaShotsberger-Gray@hmhs.com]
>> >Sent:	Tuesday, August 31, 1999 7:39 PM
>> >To:	'Histonet'; t.hacker@har.mrc.ac.uk
>> >Subject:	RE: Declere
>> >
>> >Terry, We used this at Harris (and still do for some ABs).  It 
>>works
>> >quite
>> >well!  We have used both pressure cooker and Energy Beam Microwave
>> >protocols, both work well.  Give it atry.  I think you will likeit.
>> >Wanda Shotsberger
>> >Harris Methodist Hospital
>> >Fort Wort TX
>> > ----------
>> >From: t.hacker@har.mrc.ac.uk
>> >To: 'Histonet'
>> >Subject: Declere
>> >Date: Wednesday, August 25, 1999 7:41AM
>> >
>> >I have just been handed some information on a substance called
>> >Declere which apparantley de-waxes, rehydrates and unmasks
>> >antigens in formalin fixed paraffin section,all at the same time.
>> >Has anyone used, or is using this for ICC?
>> >Terry.
>> >Terry Hacker,
>> >Medical Research Council,
>> >Harwell,
>> >Oxfordshire,
>> >U.K.
>> >
>>
>>Marsha Price HT (ASCP), QIHC (ASCP)
>>
>>
>>
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>
>
>