Re: Re Eosin
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From: | "Gary W. Gill" <garywgill@email.msn.com> |
To: | Christine Lee <C.Lee@Mailbox.uq.edu.au>, Histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 07 Sep 1999 21:44:48 -0500 |
Content-Type: | |
The late Don Myers' formula is:
Biebrich scarlet (ws) (C.I. No. 26905) 0.4 gm
Eosin Y, (C.I. No. 45380) 5.0 gm
Phloxine B (C.I. No. 45410) 2.1 gm
95% ethanol 200 mL
Distilled water 800 mL
The weights given are for total dye content. Greater amounts, therefore,
must be added for dyes having less than 100% dye content (i.e., all).
Biebrich scarlet is not certified by the Biological Stain Commission; no
percent dye content will be stamped on its label. Acetic acid is not added.
Combine the ingredients at room temperature, stir, and filter before using.
Staining time is a few dips.
Bob Richmond describes it as "an eyeblasting eosin-mix" that "stained all
kinds of stuff you can't see with the wimp eosin that's around nowadays."
Bob is a Hopkins-trained pathologist who used to look at tissues stained by
this formula.
Gary W. Gill
----- Original Message -----
From: Christine Lee <C.Lee@Mailbox.uq.edu.au>
To: <Histonet@Pathology.swmed.edu>
Sent: September 07, 1999 6:42 PM
Subject: Re Eosin
> Could Gary please share Meters Eosin with us all. I am very happy with
our
> eosin but am always looking to improve things. Thanks
> Christine Lee,
> Senior Scientific officer,
> Veterinary Pathology and Anatomy,
> University of Queensland.
> C.lee@mailbox.uq.edu.au
>
>
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