Rose,
Do you rinse in tap water after staining or deionized?  Tap will wash out
the stain.  Good Luck
Jan Mahoney
Omaha, Nebraska  GO HUSKERS!
At 10:14 AM 9/13/99 -0700, Rose_Bellantoni@integra-ls.com wrote:
>Dear Histonetters,
>
>I am having a big problem with the Methyl Green Counterstain and could
>really use your help.  It works beautifully for about one week and then it
>just stops working.  No matter how long I stain it, I get only a faint
>green stain, even if I use heat.  I have used both commercial and home made
>Methyl Green with the same results.
>
>Has any one else had this problem, and if so,  do you know what caused it
>and how to fix it?
>
>If you need more information please let me know.
>
>Thanks so much.
>
>Rose Bellantoni
>
>Integra Life Sciences, CRC
>San Diego, CA
>tel:  858-622-2740
>fax: 858-535-8269
>
>
>
>