Dear Stan,

Sorry, Sodium iodite should read sodium iodate.

> Try the following:
> 
> GARVEY'S MODIFIED MAYER'S HAEMATOXYLIN
> 
> NOTES:
> This solution is a progressive haematoxylin that uses 10% less alum. Chloral hydrate, because of its toxicity, has been replaced with ethanol, a more effective penetrator and bacteriostat. Mercury ox> 
> SOLUTION:
> Dissolve 45g ammonium or potassium alum in 900ml distilled water with the aid of heat.
> Dissolve 2.5g Haematoxylin  in 100ml absolute ethanol. 
> Combine above solutions and add 0.2g Sodium Iodite and 1g citric acid.
> Mix well. The solution is stable for several months.
> STAINING:
> 1.	Dewax and hydrate sections.
> 2.	Stain in modified Mayer's Haematoxylin 40secs.
> 3.	Wash in warm water 5min.
> 4.	If required, counterstain in eosin solution.
> 5.	Dehydrate, clear and mount.
> 
> REFERENCE:
> Garvie, W., (1991) J. Histotechn 14(3):164-165.
 
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA

http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html