Re: Coated/treated Slides.

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From:Michael Stumm <>
To:Bruce Abaloz <>
Date:Thu, 02 Sep 1999 09:31:02 +0200
Content-Type:text/plain; charset="us-ascii"

For immunohistochemistry you can take either Polylysine or Superfrost Plus
slides. Especially if you work with antigen retrieval protocolls on
paraffin sections tissue will not detach. The costs for polylysine slides
or superfrost plus slides, at least in Switzerland, are the same. For In
situ hybridization we used the same slides. Not regarding the success of
the ISH the tissue will stick to the slide which is pretty much important.
I hope this infos help you.

>I was recently asked about treated/coated slides & which ones were best
>for IHC,
>In-situ work etc.etc. & as we have a selection IE: Histogrip; Poly-L-Lysine;
>Silanized/Silane treated & Superfrost Plus I am wondering what
>advice/experience Histonetters can give me?! Preferably, I would like to
>narrow the selection I offer in this lab. to one/two that will cover
>all/most needs. Thanking you all in advance, Cheers,Bruce in OZ.
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Michael Stumm, MD, Lab 405
Department of Research
Kantonsspital Basel
Hebelstrasse 20
4031 Basel
phone: ++41/61/265 23 84

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