Re: Cellusolve Help

<< Previous Message | Next Message >>
From:"J. A. Kiernan" <>
To:JoyceAnn Horner <>
Date:Thu, 09 Sep 1999 20:12:59 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Thu, 9 Sep 1999, JoyceAnn Horner wrote:

> I want to thank the nice people who responded to my first request as to where to purchase Cellusolve.  Does anyone have any information to share on how to use it. The visiting fellow in our lab who does the immunohistochemistry
> would like some ideas.  He would be using the cellusolve to dissolve 
> celloidin from temporal bone sections. He especially would like to know if the cellusolve needs to be diluted or used straight and how long should the slides be immersed in cellusolve and any other helpful bits of information that would get him started.

  This would be rather an unusual use for cellosolve.
  The usual solvent for celloidin is a 50/50 mixture
  of ether and absolute alcohol. You can use anaesthetic-grade
  ether, which contains stabilizers to delay the formation
  of peroxides. It is, of course, still highly flammable.

  It is often convenient to stain big celloidin sections
  unmounted, without removing the embedding medium. (It doesn't
  stop the stain diffusing, though it can become stained
  itself.) The best place to find detailed instructions is
  an older textbook (if you don't have an older colleague,
  which is better). There are various ways to go about staining
  and mounting these sections.
                                        John Kiernan
                                        London, Canada.

<< Previous Message | Next Message >>