Re: Cellusolve Help
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | JoyceAnn Horner <joyceann+@pitt.edu> |
Reply-To: | |
Date: | Thu, 09 Sep 1999 20:12:59 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Thu, 9 Sep 1999, JoyceAnn Horner wrote:
> I want to thank the nice people who responded to my first request as to where to purchase Cellusolve. Does anyone have any information to share on how to use it. The visiting fellow in our lab who does the immunohistochemistry
> would like some ideas. He would be using the cellusolve to dissolve
> celloidin from temporal bone sections. He especially would like to know if the cellusolve needs to be diluted or used straight and how long should the slides be immersed in cellusolve and any other helpful bits of information that would get him started.
This would be rather an unusual use for cellosolve.
The usual solvent for celloidin is a 50/50 mixture
of ether and absolute alcohol. You can use anaesthetic-grade
ether, which contains stabilizers to delay the formation
of peroxides. It is, of course, still highly flammable.
It is often convenient to stain big celloidin sections
unmounted, without removing the embedding medium. (It doesn't
stop the stain diffusing, though it can become stained
itself.) The best place to find detailed instructions is
an older textbook (if you don't have an older colleague,
which is better). There are various ways to go about staining
and mounting these sections.
John Kiernan
London, Canada.
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