Re: Cellusolve Help

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From:Sarah Christo <>,
Date:Thu, 09 Sep 1999 16:02:26 -0500
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Dear Joyce,
   I have a reference from a workshop I took where an alcohol-ether nixture is used to remove the celloidin before staining.  A text book I have has it being removed with equal parts of absolute alcohol and ether for 5-10 minutes.  

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

>>> JoyceAnn Horner <> 09/09 3:04 PM >>>
I want to thank the nice people who responded to my first request as to where to purchase Cellusolve.  Does anyone have any information to share on how to use it. The visiting fellow in our lab who does the immunohistochemistry
would like some ideas.  He would be using the cellusolve to dissolve 
celloidin from temporal bone sections. He especially would like to know if the cellusolve needs to be diluted or used straight and how long should the slides be immersed in cellusolve and any other helpful bits of information that would get him started.

Thank you for any help you can give,

Joyce Horner
Otopathology research Lab
University of Pittsburgh

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