Michael,

We just used Tris Buffer at ph10 in the microwave. You will need to figure 
out your own timing but the trick is to boil for 20 mins. No real trick here 
it's just a matter of being able to open up those determinents. I have not 
tried other methods but I'm sure other methods would work as well if not 
better. When I spoke with a tech at Pharmigen thay told me they haven't been 
able to get it to work on formalin fixed tissue...Hmmmmm!!! We decided to 
give it a shot and from what we can tell there is some real staining. Good 
Luck!

David