RE: Re: Soap deparaffinizing
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|From:||margaret blount <Margaret.Blount@unilever.com>|
|To:||marsha r price <firstname.lastname@example.org>, "email@example.com" <firstname.lastname@example.org>|
|Date:||Mon, 06 Sep 1999 09:33:21 +0100|
I would be interested in hearing your technique if you are willing to share it.
I appreciate the willingness of all contributors to help each other with
new/improved techniques whether "home made" or proprietory.
From: marsha r price [SMTP:email@example.com]
Sent: Saturday, September 04, 1999 6:36 AM
Cc: firstname.lastname@example.org; email@example.com
Subject: Re: Soap deparaffinizing
I am merely sharing information to my fellow histonetters as we do on a
daily basis, that may be useful and economical. Many histonetters have
shared their techniques and recipes with me free of charge and I will do
the same for them. Again as I have stated this is a technique that has
been published in the Journal of applied Immunohistochemistry, and is
information that is available to all that wants it. I do not question
that Declere is patent pending, however, the knowledge is there for
anyone who wants it and I will provide my technique to anyone who
requests it as they have so generously done so for me in the past and I
welcome the challenge. I do not know the proprietary ingredients of
Declere, nor do I want to know. I can only share a tried and proven
published technique with my fellow histonetters.
On Fri, 03 Sep 1999 17:57:34 -0500 cmcorp <firstname.lastname@example.org> writes:
>The formula for Declere is proprietary and confidential at this time
>Declere is patent pending. It appears to be a matter of speculation by
>Price as to its composition.
>We at Cell Marque would invite comparison of results using whatever
>formulation one may care to mix. Our own experience suggests that
>concentration of reagents in the retrieval solutions is critical to
>I would suggest that those who are interested, do there own studies
>comparing Declere versus M. Price's formulation.
>Mike Lacey, M.D.
>Cell Marque Corp.
>At 10:31 AM 9/3/99 -0500, you wrote:
>>You can just add a little soap in your Citrate Buffer if you would
>>I have used Tween 20, but it works just as well without the soap if
>>rinse with Dawn and Di water after your antigen retrieval step. That
>>all DeClere is is Citrate Buffer and Soap. Make your own and save
>>money. I will attach my procedure as an e-file when I get home, I
>>do it from work.
>>On Fri, 03 Sep 1999 08:00:32 -0400 (EDT) Tim Morken
>>>A soap deparaffinizing technique was detailed about two years ago in
>>>Medicine by a person in Sweden. What is the reference in J Appl
>>>----Original Message Follows----
>>>From: marsha r price <email@example.com>
>>>CC: WandaShotsberger-Gray@hmhs.com, Histonet@pathology.swmed.edu,
>>>Subject: Re: RE: Declere
>>>Date: Thu, 02 Sep 1999 19:01:42 -0500
>>>There is an interesting article in Journal of Applied
>>>Immunohistochemistry, that describes a time saving technique in
>>>can skip the deparaffinization step and go straight to the antigen
>>>retrieval solution. You can use any antigen retrieval solution
>>>Buffer is my favorite). It is the heat that in the antigen retrieval
>>>(HIER) that is deparaffinizing the slides. You can remove any
>>>paraffin by rinsing in Dawn dishwashing detergent and DI water after
>>>antigen retrieval step. If you would like my procedure or any
>>>Marsha Price, HT,QIHC (ASCP)
>>>On Thu, 2 Sep 1999 08:54:41 +0100 "margaret blount"
>>> >Where does this interesting reagent come from? I would like to
>>> >a whirl.
>>> >Margaret BLount
>>> >Unilever Research
>>> >-----Original Message-----
>>> >From: Shotsberger-Gray, Wanda
>>> >Sent: Tuesday, August 31, 1999 7:39 PM
>>> >To: 'Histonet'; firstname.lastname@example.org
>>> >Subject: RE: Declere
>>> >Terry, We used this at Harris (and still do for some ABs). It
>>> >well! We have used both pressure cooker and Energy Beam
>>> >protocols, both work well. Give it atry. I think you will
>>> >Wanda Shotsberger
>>> >Harris Methodist Hospital
>>> >Fort Wort TX
>>> > ----------
>>> >From: email@example.com
>>> >To: 'Histonet'
>>> >Subject: Declere
>>> >Date: Wednesday, August 25, 1999 7:41AM
>>> >I have just been handed some information on a substance called
>>> >Declere which apparantley de-waxes, rehydrates and unmasks
>>> >antigens in formalin fixed paraffin section,all at the same time.
>>> >Has anyone used, or is using this for ICC?
>>> >Terry Hacker,
>>> >Medical Research Council,
>>>Marsha Price HT (ASCP), QIHC (ASCP)
>>>Get Your Private, Free Email at http://www.hotmail.com
Marsha Price HT (ASCP), QIHC (ASCP)
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