RE: Re: Soap deparaffinizing

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From:margaret blount <Margaret.Blount@unilever.com>
To:marsha r price <mprice26@juno.com>, "cmcorp@cellmarque.com" <cmcorp@cellmarque.com>
Reply-To:
Date:Mon, 06 Sep 1999 09:33:21 +0100
Content-Type:TEXT/PLAIN; CHARSET=US-ASCII

Marsha,

I would be interested in hearing your technique if you are willing to share it.
 I appreciate the willingness of all contributors to help each other with
new/improved techniques whether "home made" or proprietory.
Thanks
Margaret

-----Original Message-----
From:	marsha r price [SMTP:mprice26@juno.com]
Sent:	Saturday, September 04, 1999 6:36 AM
To:	cmcorp@cellmarque.com
Cc:	histonet@pathology.swmed.edu; mprice26@juno.com
Subject:	Re: Soap deparaffinizing

I am merely sharing information to my fellow histonetters as we do on a
daily basis, that may be useful and economical. Many histonetters have
shared their techniques and recipes with me free of charge and I will do
the same for them. Again as I have stated this is a technique that has
been published in the Journal of applied Immunohistochemistry, and is
information that is available to all that wants it. I do not question
that Declere is patent pending, however, the knowledge is there for
anyone who wants it and I will provide my technique to anyone who
requests it as they have so generously done so for me in the past and I
welcome the challenge. I do not know the proprietary ingredients of
Declere, nor do I want to know. I can only share a tried and proven
published technique with my fellow histonetters.

Marsha Price



On Fri, 03 Sep 1999 17:57:34 -0500 cmcorp <cmcorp@cellmarque.com> writes:
>Dear Histonetters:
> 
>The formula for Declere is proprietary and confidential at this time 
>since
>Declere is patent pending. It appears to be a matter of speculation by 
>Ms.
>Price as to its composition. 
>
>We at Cell Marque would invite comparison of results using whatever
>formulation one may care to mix. Our own experience suggests that
>concentration of reagents in the retrieval solutions is critical to 
>the
>results obtained. 
>
>I would suggest that those who are interested, do there own studies
>comparing Declere versus M. Price's formulation. 
>
>Mike Lacey, M.D. 
>Medical Director 
>Cell Marque Corp.
>
>At 10:31 AM 9/3/99 -0500, you wrote:
>>Tim, 
>>You can just add a little soap in your Citrate Buffer if you would 
>like.
>>I have used Tween 20, but it works just as well without the soap if 
>you
>>rinse with Dawn and Di water after your antigen retrieval step. That 
>is
>>all DeClere is is Citrate Buffer and Soap. Make your own and save 
>mega
>>money. I will attach my procedure as an e-file when I get home, I 
>cannot
>>do it from work.
>>Marsha Price
>>
>>On Fri, 03 Sep 1999 08:00:32 -0400 (EDT) Tim Morken 
><timcdc@hotmail.com>
>>writes:
>>>Marsha,
>>>
>>>A soap deparaffinizing technique was detailed about two years ago in 
>
>>>Lab 
>>>Medicine by a person in Sweden. What is the reference in J Appl 
>>>Immuno?
>>>
>>>
>>>----Original Message Follows----
>>>From: marsha r price <mprice26@juno.com>
>>>To: Margaret.Blount@unilever.com
>>>CC: WandaShotsberger-Gray@hmhs.com, Histonet@pathology.swmed.edu, 
>>>T.Hacker@har.mrc.ac.uk
>>>Subject: Re: RE: Declere
>>>Date: Thu, 02 Sep 1999 19:01:42 -0500
>>>
>>>Margaret,
>>>There is an interesting article in Journal of Applied
>>>Immunohistochemistry, that describes a time saving technique in 
>which 
>>>you
>>>can skip the deparaffinization step and go straight to the antigen
>>>retrieval solution. You can use any antigen retrieval  solution 
>>>(Citrate
>>>Buffer is my favorite). It is the heat that in the antigen retrieval 
>
>>>step
>>>(HIER) that is deparaffinizing the slides. You can remove any 
>>>residual
>>>paraffin by rinsing in Dawn dishwashing detergent and DI water after 
>
>>>the
>>>antigen retrieval step. If you would like my procedure or any 
>recipes 
>>>let
>>>me know.
>>>
>>>Marsha Price, HT,QIHC (ASCP)
>>>
>>>On Thu, 2 Sep 1999 08:54:41 +0100 "margaret blount"
>>><Margaret.Blount@unilever.com> writes:
>>> >Where does this interesting reagent come from? I would like to 
>give 
>>>it
>>> >a whirl.
>>> >Thanks,
>>> >Margaret BLount
>>> >Unilever Research
>>> >Beds
>>> >margaret.blount@unilever.com
>>> >
>>> >-----Original Message-----
>>> >From:	Shotsberger-Gray, Wanda
>>> >[SMTP:WandaShotsberger-Gray@hmhs.com]
>>> >Sent:	Tuesday, August 31, 1999 7:39 PM
>>> >To:	'Histonet'; t.hacker@har.mrc.ac.uk
>>> >Subject:	RE: Declere
>>> >
>>> >Terry, We used this at Harris (and still do for some ABs).  It 
>>>works
>>> >quite
>>> >well!  We have used both pressure cooker and Energy Beam 
>Microwave
>>> >protocols, both work well.  Give it atry.  I think you will 
>likeit.
>>> >Wanda Shotsberger
>>> >Harris Methodist Hospital
>>> >Fort Wort TX
>>> > ----------
>>> >From: t.hacker@har.mrc.ac.uk
>>> >To: 'Histonet'
>>> >Subject: Declere
>>> >Date: Wednesday, August 25, 1999 7:41AM
>>> >
>>> >I have just been handed some information on a substance called
>>> >Declere which apparantley de-waxes, rehydrates and unmasks
>>> >antigens in formalin fixed paraffin section,all at the same time.
>>> >Has anyone used, or is using this for ICC?
>>> >Terry.
>>> >Terry Hacker,
>>> >Medical Research Council,
>>> >Harwell,
>>> >Oxfordshire,
>>> >U.K.
>>> >
>>>
>>>Marsha Price HT (ASCP), QIHC (ASCP)
>>>
>>>
>>>
>>>______________________________________________________
>>>Get Your Private, Free Email at http://www.hotmail.com
>>>
>>
>>
>>
>

Marsha Price HT (ASCP), QIHC (ASCP)




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