RE: Processing Problem???

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From:Cynthia Favara <>
To:"'Cynthia A Delong'" <DELONG_CYNTHIA_A@LILLY.COM>,
Date:Tue, 14 Sep 1999 10:52:15 -0400
Content-Type:text/plain; charset="iso-8859-1"

	I do a lot of work on mouse tissue especially brain. I have had the
same problem at times and here are my suggsetions:
	1.Try slightly different angle when cutting and float on a lower
temperature water bath for longer. I mean a bath temp as low as 35C and
floating for up to 10 minutes.
	2. How thick are you cutting? If tissue section thickness is not a
major issue might want to try increase or decrease. I generally find thicker
sections more problematic in terms of wrinkles.
	3. If all else fails I would reverse the processing  ie take back
though clearing agents to alcohol and reprocess. I have done this before
when the processor got started on the wrong station.
	4. Fix yourself a good margarita and tell your pathologist there are
always more mice!!

My best guess is that the specimens were not processed in the usual manner.
Best of luck - feel free to contact me via e-mail or phone if you would like
more help.
Cynthia Favara
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286

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