I would use Azure B at different ph.
0.2% ph 1.0 will stain cartilage only
0.2% ph 4 will stain cartilage and bone
0.2% ph 7 will stain all tissue components
Patsy Ruegg

-----Original Message-----
From: John Howes [mailto:john.howes@stonebow.otago.ac.nz]
Sent: Tuesday, August 31, 1999 3:10 PM
To: histonet@pathology.swmed.edu
Subject: Ossification


Thank you everybody who replied to my inquiry on staining the foot of a 10
- 11 day feotal rat. However the question that I posed may not have been
clear enough. I do not wish to contrast bone and cartilage in whole mounts.
I need to demonstrate the degenerating tissue between the developing bones
in the foot at a cytological level.