Processing Problem???

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Date:Mon, 13 Sep 1999 13:57:25 -0500
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Hello all,

I am in desperate need of information.  I have some mouse brain tissue that
will not cut.

I have used the same processing schedule for every process and I have 1 batch
of animals that are not cutting.  Low and behold it is the 1 batch that the
pathologist want.

Anyway, When I cut them and place them on a 37 degree water bath (as I have
always) the tissue wrinkles ups and pulls away from the paraffin.

I have tried to reembed them and it seemed to help a little but not enough
considering I have to serial section through the whole piece.

If there are any ideas of what may have caused the problem or any solutions
PLEASE contact me via e-mail or phone.  This is very important work and I have
not had alot of experience with this type of problem.

Thank all of you

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