IPEX PROBLEMS

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From:"Mitchell, Jeannette M." <Jeannette.Mitchell@vtmednet.org>
To:"'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
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Date:Sun, 12 Sep 1999 12:49:47 -0400
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> -----Original Message-----
> From:	Mitchell, Jeannette M. [SMTP:Jeannette.Mitchell@vtmednet.org]
> Sent:	Saturday, September 11, 1999 12:52 PM
> To:	'histonet@pathology.swmed.edu'
> Subject:	ippexes
> 
> Dear fellow histotechs,
> 
> Our lab is having problems with mostly cytolgy ipexes.  The negative and
> positive patient control is staining in the lymphocytes. The tumor cells
> are staining.  There is also nonspecific staining in the tumor. On the
> negative surgical tissues we are also seeing a blush and staining at the
> edges of the tissues.
>    We are currently using the Dako lsab+ kit,  we are blocking with
> methanol/hydrogen perxiode and using avidin/biotin  on these thin preps.
> I discovered an insert in the Dako kit that mentions that there has been
> minor modifications in the link component and that it would affect only
> goat primary antibodies.  Has anyone experienced any of the above
> problems?? Please if you let me know.  I welcome any theories one may
> have.  We have been doing ipexes on the dako machine for quite a while and
> have had no problems.
> 
> Jeannette
>  



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