H-Ras
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From: | "Mcleod, H, Heather" <HMCLEOD@chempath.uct.ac.za> |
To: | HISTONET@pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 08 Sep 1999 13:32:17 -0002 |
Content-Type: | |
Dear Histonetters
I need help, help, help.
I have to do IHC using the H-Ras (F235): sc-29 ab. The antibody
was purchased from Santa Cruz Biotechnology, and the material is
FFPE breast tissue. I would like to know what to use to control the
procedure, what sort of antigen retrieval and if anybody has actually
used this particular ab, what dilution to start with. I have made
an unsuccessful attempt.......using the pressure cooker for
ag retrieval, breast tumour tissue and an ab concentration of 5ug/ml.
The result was not very encouraging and would appreciate some advice
before the next run. Otherwise, it is a waste of time and money.
I read so much good advice and learnt so much on the histonet,
and am therefore sure that someone will be able to help, so thankyou
very much.
Heather
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