AW: Cytoplamic staining in P53 and Ki-67

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From:"Nader, Alexander" <>
To:"'Dave Tacha'" <>, "" <>
Date:Fri, 03 Sep 1999 05:01:54 -0100
Content-Type:text/plain; charset=iso-8859-1

> Also has anyone ever seen Ki-67 (MIB-1 or other Ki-67
> monoclonal antibodies) with both cytoplasmic and nuclear >staining?  Is
there a remedy?

MIB-1 sometimes faintly stains the cytoplasm too, without any
background-staining. IMHO it's more often when EDTA as HIER-buffer is used.
But there are also other nuclear antibodies which sometimes are reactive
with the cytoplasm too like estrogen-receptor.

Has anybody an explanation why sometimes antibodies (especially CD20, clone
L26 from DAKO) stain nucleoli (of all cells, not only the expected
target-cells) very well?

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