AW: Cytoplamic staining in P53 and Ki-67
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| From: | "Nader, Alexander" <alexander.nader@exchange01.wgkk.sozvers.at> |
| To: | "'Dave Tacha'" <dtacha@ncal.verio.com>, "histonet@Pathology.swmed.edu" <histonet@pathology.swmed.edu> |
| Reply-To: | |
| Date: | Fri, 03 Sep 1999 05:01:54 -0100 |
| Content-Type: | text/plain; charset=iso-8859-1 |
> Also has anyone ever seen Ki-67 (MIB-1 or other Ki-67
> monoclonal antibodies) with both cytoplasmic and nuclear >staining? Is
there a remedy?
MIB-1 sometimes faintly stains the cytoplasm too, without any
background-staining. IMHO it's more often when EDTA as HIER-buffer is used.
But there are also other nuclear antibodies which sometimes are reactive
with the cytoplasm too like estrogen-receptor.
Has anybody an explanation why sometimes antibodies (especially CD20, clone
L26 from DAKO) stain nucleoli (of all cells, not only the expected
target-cells) very well?
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