RE: [Histonet] PLS Help with IHC protocol

From:"Patsy Ruegg"

We need to know what the concentration of the antibody is before you make it

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
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-----Original Message-----
[] On Behalf Of Kimberly
Sent: Tuesday, September 30, 2008 9:25 AM
Subject: [Histonet] PLS Help with IHC protocol

Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block

Can someone break this down for me in simple instructions? 

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 


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