RE: [Histonet] Freezing tissues

From:"Robyn Vazquez"

Just a thought, could you use a  clear water soluble microbiology gelatinous plate?  Never done this, but it would keep them standing on edge.


-----Original Message-----
From: [] On Behalf Of Bascaramurty, Saro
Sent: Monday, September 29, 2008 9:54 AM
Subject: [Histonet] Freezing tissues


Does anyone out there have a good method to freeze animal vessels in order to have good morphology/free of artefacts etc. for some spectroscopic studies and histochemistry? Using liquid nitrogen is ok, but not isopentane or any other chemicals that would interfere with the spectra. Thanks in advance for any input that would improve our freezing method.

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