[Histonet] paraffin sectioning problems

From:James Dooley



I recently started paraffin sectioning and I am having trouble with getting good quality sections reproducibly. I was wondering if you have any tips that may help. I am sectioning thymus, pancreas, and lung. Section thickness is 10 um. The microtome that I am using is Reichert-Jung Supercut, disposable knife holder by Leica, and Accu-Edge low profile blades. I fix the tissue in a modified Carnoy's fix overnight, dehydrate in graded alcohol, 100% ETOH, Toluene 2x15 minutes, Paraffin at 65 degrees overnight with 2X changes of paraffin. Embed and store at RT. The paraffin I am using is Fisherbrand Paraplast X-tra Tissue embedding medium. Any suggestion would be greatly appreciated. 

Thank you in advance,
James Dooley


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>